Overview
The harmful effects of drugs and physical stressors on cells are measured through cytotoxicity assays. C.LIVE Tox reagents employ a straightforward mix-and-read procedure to assess cytotoxicity in real-time.
Image Credit: CYTENA GmbH
C.LIVE Tox Green and Red are both nonpermeable to living cells and nonfluorescent on the outside. The reagent produces intense fluorescence after attaching to DNA and passing through broken plasma membranes to penetrate dead cells. The addition of C.LIVE Tox reagents had no impact on the growth or morphology of healthy cells.
Image Credit: CYTENA GmbH
Advantages of CYTENA cytotoxicity assays
- Simple mix-and-read workflow
- Direct detection of dead cells with C.LIVE Tox reagents
- Kinetic monitoring of cytotoxicity by measuring fluorescent objects over time
- Designed for long-term live cell studies
Mix-and-read workflow
Perform the cytotoxicity assays in three easy steps: Plate the cells, add compounds using C.LIVE Tox reagent, and use the CELLCYTE X imaging system to kinetically evaluate cell health.
Image Credit: CYTENA GmbH
Real-time cytotoxicity analysis in the incubator
Without taking the cells out of the incubator, use the CELLCYTE X to image and analyze the fluorescent signals of the cytotoxicity assay.
Image Credit: CYTENA GmbH
HeLa cells were treated with increasing concentrations of Staurosporine. C.LIVE Tox Green was added to each well (250 nM) and time-lapse images were obtained with the CELLCYTE X. The control well shows minimal green signal compared to Staurosporine-treated well at 48 hours (left panel). Automated quantification shows the time- and dose-dependent toxicity effects of Staurosporine (right panel). Image Credit: CYTENA GmbH
Applications
- Measure the toxic effects on cells from drugs and physical stressors
- Kinetic monitoring of cytotoxicity
C.LIVE Tox reagents product information
Source: CYTENA GmbH
Image Credit: CYTENA GmbH