Immunohistochemistry Analysis of a Living Skin Equivalent

Labskin displays immunohistochemistry markers that are relatively analogous to human skin. It can be used with conventional paraffin wax embedding and cryo-sectioning preparation techniques, as well as a wide range of staining and immunohistochemistry protocols. The method used to make all the samples was Paraffin wax embedding.

Labskin structure analysis

Basement membrane analysis.

Basement membrane analysis. Image Credit: Labskin.

Barrier function analysis.

Barrier function analysis. Image Credit: Labskin.

Cell proliferation analysis.

Cell proliferation analysis. Image Credit: Labskin.

Labskin microbial response analysis

Cathelididin

Image Credit: Labskin.

RNase 7

Image Credit: Labskin.

HBD2

Image Credit: Labskin.

Labskin can be used within the same experimental framework to evaluate numerous endpoints simultaneously. The endpoints contain cytokine responses (such as PGE2, IL-6, IL-8, IL-10, IL-1α, TNF α, etc.), wound repair, histological changes, and photo-reactivity. Endpoints like skin commensal and pathogenic microorganisms can also be evaluated.

About Labskin

At Labskin we deliver human skin microbiology services to support R&D activities in the cosmetic, personal care, medical device, and pharmaceutical sectors. With the company's sector experience and use of technology, customers will be accessing industry-focused services supported by world-leading skin science expertise.

Whether customers need rapid, focused analysis or flexible, tailor-made research programs, the company can help in developing and validating skincare ingredients and products that really work. Labskin's skin model is a 3D human skin equivalent that incorporates vital biological components to model normal skin function.

Developed over 12 years with more than 30 scientific journal publications, it is made from young keratinocytes (human skin cells) and adult fibroblasts (metabolically-active, collagen-producing human skin cells).

“An ideal platform for basic or applied skin research, testing compounds or formulated products for the cosmetic, pharmaceutical and chemical sectors.”


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Last updated: Feb 28, 2020 at 11:53 AM

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