Automate Pipetting With OT-2

The bench-top liquid handler OT-2 is easily accessible and adaptable enough to automate many standard applications.

Video Credit: Opentrons

Automate the Most Time-Consuming Applications in the Lab

General Liquid Handling

Image Credit: Opentrons

The easiest method to save time in the lab. The OT-2 pipetting robot makes it easy for users to automate various fundamental liquid handling operations, including sample pooling, cherry picking, serial dilution, tube and microplate filling, and normalization. Users can design their own protocols, ask the Applications Engineering team to build one, or select from a variety of tested protocols in the Protocol Library.

PCR Setup

Image Credit: Opentrons

Another widely used automation application, particularly for users preparing many samples. To mix samples and PCR mastermix in the reaction plate placed in the thermocycler or qPCR equipment, users can utilize the OT-2 pipetting robot.

Protein Purification

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The most cost-effective method for automating small-scale protein purification, proteomics sample processing, and enzymatic and chemical lysis. Opentrons currently provides two techniques for automating the OT-2 pipetting robot: dual-flow chromatography-based automation and magnetic bead-based automation.

Small Footprint, Big Impact

Tech Specs

Source: Opentrons

Dimensions	63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (W,D,H) Takes up half of your standard lab bench
Weight	48 kg or 105.8 lb
Pipette configurations	Single- and 8-channel pipetting 2 pipette mounts for a configuration of 1 or 2 single- or 8-channel pipettes. Pipettes are easily interchangeable.
Pipette volumes and specs	Single-channel: 1-1000 µL 8-channel: 1-300 µL
Deck	11 deck slots enable countless configurations Deck slots are compatible with standard SBS dimensions. Deck also includes a removable trash bin.
Connectivity	WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0
Video	Integrated video camera (Included for future or custom functionality)
Audio	Integrated audio system (Included for future or custom functionality)
Power requirements	Robot Power Input: 36 VDC, 6.1 A Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC For additional power requirement information, please review this support article
Frame composition	Rigid steel and CNC aluminum design
Operating temperature & relative humidity	Recommended 20-24 °C Recommended 40-60% RH
Time to fill 96-well plate	Pipette Distribute, one tip Transfer, same tip Transfer, new tip P20 8-Channel 0:22 1:35 4:19 P300 8-Channel 0:26 1:38 4:24 P20 Single-Channel 3:12 13:10 36:01 P300 Single-Channel 4:01 13:39 41:31 P1000 5:54 15:21 44:50
Single-channel accuracy & precision	Pipette Volume Random Error Systematic Error     %D µl %CV µl P20 1 10 20 ±15% ±2% ±1.5% 0.15 µl 0.2 µl 0.3 µl ±5% ±1% ±0.8%   P300 20 150 300 ±4% ±1% ±0.6% 0.8 µl 1.5 µl 1.8 µl ±2.5% ±0.4% ±0.3%   P1000 100 500 1000 ±2% ±1% ±0.7% 2.0 µl 5.0 µl 7. 0µl ±1% ±0.2% ±0.15%
8-channel accuracy & precision	Pipette Volume Random Error Systematic Error     %D µl %CV µl P20 1 10 20 ±20% ±3% ±2.2% 0.2 µl 0.3 µl 0.44 µl ±10% ±2% ±1.5%   P300 20 150 300 ±10% ±2.5% ±1.5% 2.0 µl 3.75 µl 4.5 µl ±4% ±0.8% ±0.5%
Sterility	Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment Add the Opentrons HEPA Module, or add your own UV lights
Viscosity	For best practices on automating viscous liquid handling, review our application note Viscous Liquid Handling Automation using Opentrons OT-2 ›
API compatibility	Interfaces with any product that has an API Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.
Interface	Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later. Opentrons App
Tip compatibility	Use Opentrons Tips and Opentrons Filter Tips Opentrons guarantees the performance of Opentrons Tips specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly, so third-party tip Opentrons cannot guarantee performance.
Labware compatibility	Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, you can add it using our Labware Creator or our team.
Warranty	One-year warranty against manufacturer defects Stress tested to last a minimum of 3 years of full-time use
Return policy	60 days, 100% risk-free
Certifications	Certifications complete (CE, FCC, NRTL, CB, ISO 9001) Not certified/validated (IVD, GMP)
Example Commands	Tip Handling Pick up tip Drop tip Return tip New tip (always, never, once) More… Liquid Control Aspirate Dispense Aspirate speed Dispense speed Blowout Touch tip Mix Air gap Utility Move to Delay User-specified pause Homing Comment Transfer Basic transfer Volumes > Pipette Volume Distribute Consolidate Variable Settings Speed Volume Count Other Dispose
What’s in the box	OT-2 robot Calibration block Removable block Trash container Quickstart guide Power supply and regional IEC cable Ethernet cable Ethernet-to-USB dongle Tool kit

Getting Started

Everything users require, including step-by-step assistance, to automate the lab.

• Demo the OT-2
  • Obtain a virtual demo of the OT-2 and get answers regarding the specific application.
• Order the OT-2
  • Bundle the OT-2 with the pipettes, hardware, and labware users require
• Receive and unbox the OT-2
  • The user can easily unbox and set up the device by following the online guide
• Custom or urgent support
  • Opentrons offers onsite installation, customized protocol development, and more

Use What the User Has, Add What They Need

Image Credit: Opentrons

Choose the Hardware and Pipettes

• 11 deck slots, plus trash, and space for two swappable, single and 8-Channel pipettes
• Magnetic, Temp, Thermocycler, Heater-Shaker, and HEPA Filter modules available

Use the Existing Labware and Reagents

• Reagent and consumables agnostic
• Compatible with any ANSI/SLAS-compliant labware

Choose the Right Protocol Method

A protocol is the code that directs the robot through the experiment. They have developed many methods for creating a protocol accessible to everyone, from automation novices to seasoned Python coders.

Custom Protocol

• Ideal for users who want a quick, sophisticated, or unique protocol
  • Have a protocol designed specifically for the experiment created by the specialists

Protocol Library

• Ideal for new users seeking to acquire experience
  • Look for open-source protocols that are thoroughly documented

Protocol Designer

• Ideal for users who wish to develop a novel protocol without having to code
  • Create new protocols using the user-friendly drag-and-drop interface

Python Protocol API

• Ideal for those who know Python
  • Using the open-source Python Protocol API, users can develop their own protocols.

Space for Two Pipettes

Image Credit: Opentrons

Reliable liquid transfers from 1–1000 µL with the swappable single- and 8-channel pipettes.

Image Credit: Opentrons

• Single-channel
  • P20
  • P300
  • P1000

Image Credit: Opentrons

• 8-channel
  • P20
  • P300