Digital Holographic Microscopy Reveals Microbial Biosignatures in Harsh Environments

New research from Portland State University focused on identifying signs of life - biosignatures - in extreme environments here on Earth. Researchers investigated whether microbial active motion (e.g., swimming), morphology and optical properties could serve as biosignatures using in situ video microscopy at a range of extreme field sites, many of which had not been previously explored with this technique. 

These environments are considered strong analogs for extraterrestrial settings, such as those found on other planets and moons in our solar system."

Carl Snyder, lead author and Ph.D. candidate in physics, Portland State

The researchers found that at least one of the three biosignatures (motion, morphology, or optical properties) was present in every environmental sample tested, ranging from hot deserts to Arctic ice and alkaline springs. This supports the idea that even in extreme environments, some fraction of microbes exhibit detectable life-indicating characteristics.

This research also highlights digital holographic microscopy (DHM) as a promising tool for future space missions analyzing liquid samples in search of life. It also emphasizes the ubiquity of microbial swimming as a potential biosignature. To explore this further, researchers introduced chemical and thermal stimuli to test their effects on microbial motility. The responses varied - some environments showed strong microbial reactions, while others showed little to none.

Despite these differences, a consistent finding across all sites was the presence of microbial biosignatures wherever was explored with DHM.