CellenONE®

Overview

CellenONE^® is an open platform for isolating single cells and dispensing nanoliters.

cellenONE^® X1

Cell morphology and/or multichannel fluorescence can be used to sort and dispense single cells using the standalone cellenONE instrument

cellenONE^® X1 BSC

For applications requiring complete sample, operator, and environmental protection, the cellenONE instrument is integrated under a class II biosafety cabinet

Features

High accuracy single cell isolation and dispensing

• CellenONE^® is a ground-breaking platform that offers high-precision single cell isolation and dispensing in real time
• It is based on sciDROP PICO precision dispensing technology and combined with advanced image processing
• CellenONE^® generates droplets using gentle acoustic waves, preserving exceptional cell viability for all cloning applications and maintaining protein expression for sequencing applications

Image Credit: Cellenion

Versatile technology for a variety of samples

• High-resolution optics make it possible to isolate any type of cell from any sample size, including mammalian cells, bacteria, fungi, archaea, and plants
• Aspirate/dispense mode operation enables high recovery of a variety of samples, ranging in size from tiny cell suspensions with just a few microliters and a few dozen cells to much larger samples with thousands of cells

Image Credit: Cellenion

Benefits

• High accuracy single cell isolation and dispensing
• Gentle on cells, ensuring outstanding cell viability
• Compatible with a wide range of particle sizes (0.5 µm–80 µm)
• Ideal for rare cell samples (zero dead volume and high recovery)
• High-throughput isolation of hundreds of cells in only a few minutes
• Selection of the cells of interest with user-defined sorting parameters (morphology and/or fluorescence markers)

Image Credit: Cellenion

Applications

Single cell omics and cell line development applications can both benefit from cellenONE^®’s low volume, high viability, and high accuracy single cell isolation systems

Single cell omics

Image Credit: Cellenion

Cell line development 

Image Credit: Cellenion

Cellenion has worked successfully with a large variety of cell types

• Cell lines
  • CHO, Hybridoma, HEK293T, HeLa, A549, PC3, H1975, HepaRG
• Nuclei
  • From cell line, fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissue slices
• Microbeads
  • PMMA microbeads from 2 to 30 µm diameters
  • TOYOPEARL^®
  • PS microbeads
• Primary cells
  • PBMC (including B- and T- cell fractions)
  • Fibroblasts, keratinocytes, melanocytes, cardiomyocytes, HUVEC
  • Neural stem cells

Image Credit: Cellenion

Specifications

Source: Cellenion