Researchers are investigating the creation of biofuel from microorganisms as a result of the increased need for sustainable fuel alternatives worldwide. Recent research conducted under the direction of Bharat Kumar Majhi offers fascinating advances in the bioengineering of cyanobacteria to produce ethanol.
The researchers’ use of genetic and metabolic alterations resulted in notable increases in ethanol output, establishing cyanobacteria as a viable option for renewable fuel.
Cyanobacteria, which are known for their photosynthesis, have the potential to produce sustainable fuel. However, their natural ethanol production rates remain modest, demanding increases. The current study sought to address this constraint by genetically altering cyanobacterial strains to optimize carbon flow and overexpress critical enzymes, resulting in large increases in ethanol production rates.
The researchers used Synechocystis sp. PCC 6803, a cyanobacterium model, and added genes coding for pyruvate decarboxylase and alcohol dehydrogenase enzymes, which are required for ethanol manufacture. Through deliberate changes, the team shifted carbon from glycogen synthesis to ethanol production, lowering carbon waste. To increase productivity, environmental modifications such as varying light and temperature conditions were implemented.
Over the course of seven to ten days, the modified cyanobacteria showed ethanol production rates ranging from 0.24 to 3.8 g/L. The modified strains efficiently partitioned carbon toward ethanol generation by reducing carbon flux toward glycogen synthesis and boosting Calvin cycle enzyme expression. Interestingly, under a variety of conditions, the modified strains consistently produced ethanol, demonstrating significant performance gains over earlier models.
These results emphasize how crucial customized metabolic and genetic approaches are when bioengineering cyanobacteria to produce fuel. Cyanobacteria can function as effective and scalable biofuel sources by rerouting carbon flow and overexpressing specific enzymes.
The concept represents a significant development in renewable energy, providing a viable alternative to fossil fuels. However, obstacles like as large-scale production, cost-effectiveness, and environmental impact persist. Future studies will focus on optimizing these parameters, opening the path for actual applications in biofuel markets.
Bharat Kumar Majhi’s study represents a significant advancement in cyanobacteria-based biofuel manufacturing. The successful genetic and metabolic engineering of Synechocystis sp. PCC 6803 reveals the viability of increasing cyanobacterial ethanol production, presenting it as a viable renewable biofuel candidate. As global energy difficulties rise, inventions like these are critical to achieving a sustainable future.
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Journal reference:
Majhi. B. K., (2024) Cyanobacteria: Photosynthetic Cell Factories for Biofuel Production. Journal of Bioresources and Bioproducts. doi.org/10.1016/j.jobab.2024.10.001.