A significant finding about the energy production process of human cells has been made by researchers at the Karolinska Institute's Department of Cell and Molecular Biology. Their study, published in the EMBO Journal, uncovers the intricate mechanisms by which mitochondria process transfer RNA (tRNA) molecules, which are crucial for energy production.
The cell's powerhouses, the mitochondria, require correctly digested tRNAs to produce proteins for energy. Serious mitochondrial illnesses can result from issues with tRNA processing. Up until today, nothing was known about the precise mechanism of tRNA maturation in mitochondria.
Our study reveals, at a molecular level, how the mitochondrial RNase Z complex recognizes and processes tRNA molecules. By using advanced cryo-electron microscopy, we have been able to visualize the complex in action, capturing snapshots of tRNA at different stages of maturation. This is a significant step forward in understanding how our cells produce energy and maintain healthy function.”
Genís Valentín Gesé, Study First Author, Karolinska Institute
A Glimpse into the Molecular Machinery
The mitochondrial RNase Z complex, which is essential for tRNA maturation, was seen by the researchers using sophisticated cryo-electron microscopy. They obtained high-resolution pictures that demonstrate the sequential processing of tRNA molecules by this complex.
Seeing the RNase Z complex in such detail is like watching the gears of a finely tuned engine. We can observe how each component interacts with the tRNA, providing us with invaluable insights into the precise mechanisms of tRNA maturation.”
Genís Valentín Gesé, Study First Author, Karolinska Institute
Unveiling the Sequential Processing Mechanism
The identification of tRNAs' 5′-to-3′ processing order, which guarantees proper preparation for protein synthesis, is one important finding. The study also describes how the RNase Z complex prevents tRNA processing errors by not cleaving tRNAs that already have their necessary 3′-CCA tail.
Understanding the directionality of tRNA processing is crucial. It ensures that the tRNA molecules are properly matured and functional, which is essential for the mitochondria to produce energy efficiently.”
Martin Hällberg, Study Senior Author, Karolinska Institute
Crucially, the study connects mitochondrial disorders to particular mutations in the ELAC2 gene. Comprehending these alterations aids in the creation of focused treatments for ailments such as intellectual disability and cardiomyopathy.
Martin Hällberg said, “By visualizing where these mutations occur and how they affect the structure and function of the RNase Z complex, we can understand the molecular basis of certain mitochondrial diseases. This knowledge is crucial for developing targeted therapies to correct or compensate for these defects.”
This discovery advances general knowledge of mitochondrial biology and opens up new avenues for the diagnosis and treatment of mitochondrial disorders.
Source:
Journal reference:
Valentín Gesé, G., et al. (2024) Structural basis of 3′-tRNA maturation by the human mitochondrial RNase Z complex. The EMBO Journal. doi.org/10.1038/s44318-024-00297-w.