The ClonePix® 2 Mammalian Colony Picker from Molecular Devices is a completely automated system for the selection of high-value clones utilized in cell line development and antibody discovery. Users can screen more clones in less time with monoclonal verification on day zero, then screen and identify the highest produces in just a matter of weeks rather than months.
Automate Antibody Discovery and Cell Line Development Workflows
ClonePix 2 picker is 10× faster compared to labor-intensive limiting dilution and FACS. The advanced software and combined robotics allow a screening speed of >10,000 clones daily.
Select Cells with Desirable Attributes With Monoclonality Assurance from Day 0
It is possible for users to seamlessly screen and select clones based on protein productivity, cell viability, antigen-specificity, and expression levels of tagged recombinant proteins.
Increase the Probability of Identifying High-Value Clones While Eliminating or Recovering Unstable Clones Early
Picking accuracy <1 mm. Robotic picking helps decrease the risk of colony disturbance. Images of picked clones can be stored with data.
ClonePix 2. Image Credit: Molecular Devices UK Ltd
Features
Multiple Detection Methods
White light determines and quantifies size, clone morphology, and proximity. Fluorescence denotes expression level and/or specificity. Comes with a maximum of five fluorescent filters for multiplexing.
Integrated Plate Storage
Consists of two storage stacks for source and destination plates, each with a capacity of 10 plates.
Animal-Free Media and Reagents
CloneMedia cell-culture media is chemically characterized and animal-free. It has been improved to increase productivity and assist in envisioning secreted antibodies while being utilized with the CloneDetect™ detection agent.
Sterility Maintenance
A host of sterility features and options, such as a UV light process for sanitizing the interior of the instrument. Pin washing and halogen drying come as standard.
Discrete Colony Formation
Semi-solid CloneMediaTM encourages single cells to grow into discreet colonies and allows for ease of plating. The media allows for a higher density of clones to be screened.
Custom Automation Options
The Advanced Workflow Engineering Solutions Team is on hand to help tailor the monoclonality system and provide added services like combined verification of monoclonality.
Redefine Clone Screening and Selection With Transformative Cell Line Development Workflows
Users can empower their team with data analysis that automatically produces a map of clones and their secretion levels from a range of images generated in situ.
The ClonePix can also be tailored to include image-based monoclonality assurance on day zero. This means that a single round of screening can be carried out, and users can then pick the highest produces in weeks rather than months.
Image Credit: Molecular Devices UK Ltd
Data Analysis and Tracking — Reveal Stable Clones Faster
Data Analysis
- Automatically produce a 2D map of clones and their secretion levels from a series of images generated in situ
- Screen and select colonies depending on:
- Ranking as per fluorescence levels
- Size, roundness, and proximity to neighbors
- Closely placed colonies neglected through user-controlled “proximity” software setting
Data Tracking
All appropriate data linked to each colony (such as images taken before and after picking together with their picking coordinates) are saved automatically for review and downstream analysis.
Image Credit: Molecular Devices UK Ltd
Enhanced With Monoclonality Assurance
The Same ClonePix Workflow, But With Improved High-Resolution Single-Cell Imaging Capability on Day 0
The improved ClonePix 2 system can automatically screen and pick clones that are both high producing and monoclonal — all in one system. More clones can also be screened in less time with monoclonal verification on day zero. The user can then screen and pick the highest produces in as little as two weeks.
- Decrease screening time from two rounds to one by offering image-based evidence of clonality.
- The quick Z-stack acquisition feature enables the detection of single cells across the medium volume, not just a single focal plane, on day 0.
- Easy workflow from single-cell identification and productivity screening with the multipurpose system.
Enhanced for Stem Cell Applications
Determine Desirable Clonal Stem Cell Colonies for High-Throughput Colony Screening and Picking
High-resolution imaging determines suitable clonal stem cell colonies for high-throughput colony screening and picking. Specialized picking pins facilitate the gentle transfer of adherent, feeder-free cells to high-density plates for clonal expansion and downstream analysis.
- Colony formation - Individual stem cells plated at a low density in 6-well plates split up and develop into colonies. Plating density is kept low to ensure that colonies come from a single parent cell.
- Clone screening - Clonally derived stem cell colonies are determined by their suitable morphological characteristics and are picked from the low-density 6-well plates and placed into higher density 96-well plates to carry forward with the screening.
The ClonePix® 2 Mammalian Colony Picker can be tailored with high-resolution optics and stem cell-specific pins, thereby enabling this established technology to be leveraged in stem cell workflows.
- Cell growth - Cell growth is determined by monitoring cell division over a given time using label-free imaging.
- Functional screening - In addition to monitoring growth, functional cell-based assays can be conducted. This can include assays assessing differentiation potential, pluripotency, ability to form 3D organoids, and other desirable traits.
Image Credit: Molecular Devices UK Ltd