Overview
C.SIGHT™ 2.0 helps users upgrade their single-cell isolation for cell line development.
The C.SIGHT 2.0 is known to be the new iteration of the single-cell dispensing system that has been specifically developed to improve cell line development (CLD) applications.
With the availability of quicker single-cell isolation processing times and the new EASY.ON cartridges for contamination-free dispensing, the C.SIGHT 2.0 offers a simple entry point into the world of single-cell handling.
The compact design of the C.SIGHT 2.0’s makes it possible to function the system in biosafety cabinets, thereby enabling single-cell isolation in barren conditions.
Features
- Quick and efficient single-cell dispensing: Single cells have been gently dispensed into a 96-well plate in just 2 minutes or into a 384-well plate in under 8 minutes
- No risk of contamination: Remove the risk of cross-contamination at the time of dispensing with single-use EASY.ON cartridges
- Assurance of clonality: Users can capture nozzle images for each dispensing run to guarantee that only single cells were dispensed in the plate
- Cell Focusing technology: Arrange cells at the center of the dispensing cartridge for much better handling and processing of fragile samples
- Easy setup: start single-cell isolation in minutes as a result of the C.SIGHT 2.0’s direct software
- Compact design: Users can place the C.SIGHT 2.0 in laminar flow hoods to separate cells in a sterile surrounding
Intuitive software
- Simple and quick setup
- Full-resolution on brightfield imaging
- Users can save all dispensing events for examination and assurance of clonality
Image Credit: CYTENA GmbH
Fast and gentle dispensing
- Separate single cells into a 384-well plate in just eight minutes
- Gently dispenses cells without asserting the cells to any sort of pressure
Image Credit: CYTENA GmbH
Eliminate cross contamination
- Gentle and safe dispensing
- No pressure on cells or liquid to develop into droplets
- No risk of cross-contamination
EASY.ON design
- Magnetically fixed cartridge for simple loading
Minimize cell loss
Add an extra layer of optimization with the patented Cell Focusing technology, which gently arranges cells at the center of the dispensing cartridge for excellent cell detection.
- Decrease cell loss
- Customized for unique or gentle cell samples
- Processing speeds can be increased
Image Credit: CYTENA GmbH
Assurance of single-cell dispensing
- Real-time imaging and processing of single-cell dispensing can be done
- Clonality probability is greater than 99%
- Nozzle images documented for each separation event
Image Credit: CYTENA GmbH
Unprecedented dispensing speed
Separation of single-cell into multi-well plates completes within minutes.
Image Credit: CYTENA GmbH
Processing times
Source: CYTENA GmbH
| |
Time |
| System boot until experiment starts |
<5 min |
| Dispensing (96-well plate) single cells |
~2 min |
| Dispensing (384-well plate) single cells |
~8 min |
| Shutdown and cleaning time |
<5 min |
Compatible with sterile biosafety cabinets
As a result of C.SIGHT’s compact size, the device can be placed within most biosafety cabinets and linked externally. The system could function from outside the biosafety cabinet through remote control terminals, thereby making the C.SIGHT 2.0’s cutting-edge technology accessible to laboratories of any size.
Image Credit: CYTENA GmbH
Applications
- Accelerating bacteria transformation and cloning workflows using microbial single-cell isolation
- Sequencing single-cell genomes of uncultivated bacteria in the human oral microbiome
- High-throughput cultivation workflow for isolation of anaerobic gut bacteria