Overview
The UP.SIGHT simplifies cell line development (CLD) workflows by automating time-consuming and laborious steps. The company has integrated the patented, highly effective, quick, and mild single-cell dispensing technology with the availability of a fast-paced, high-quality imaging system that offers a complete well image and no requirement for image stitching.
The multipurpose solution allows nozzle imaging and 3D Full Well Imaging available for double assurance of clonality from two separate optical apparatuses, thereby leading to a likelihood of clonality >99.99%.
Probability of clonality >99.99%
Throughout the world, 8 out of 10 of the best-selling drugs are developed with the help of biologics. UP.SIGHT allows users to maximize their cell line development workflow.
Double assurance of clonality
Image Credit: CYTENA GmbH
The UP.SIGHT provides two separate techniques for guaranteeing monoclonality. Initially, images are taken at the dispensing nozzle to guarantee just one cell is positioned very well, and nonclonal droplets have been rejected.
Second, the single cells are affirmed once more with the availability of 3D Full Well Imaging. It is a new and inventive technique that images the complete volume of every well from the point of dispensing with the help of the combined imager.
All-in-one solution, from single cell to colony
Image Credit: CYTENA GmbH
Image Credit: CYTENA GmbH
Image Credit: CYTENA GmbH
With the availability of a combined imager, the UP.SIGHT allows a complete single-cell cloning workflow in a single instrument. Right from mild brightfield and fluorescence based single-cell isolation to twice assured clonality to colony tracking from Day 0, the UP.SIGHT performs it all.
Featured workflow
The wide product portfolio provides a range of groundbreaking technologies and instruments for considerably increasing the quality, speed, and efficiency of users’ cell line development workflows.
Image Credit: CYTENA GmbH
Features
Higher yields, greater insights, faster processing
The UP.SIGHT integrates the patented single-cell dispensing technology with an industry-leading imaging system for an enhanced single-cell cloning workflow. This allows greater clonal yields and the processing of hundreds of clones in just 30 minutes.
Reducing the risk of cross-contamination
The UP.SIGHT’s exclusive EASY.ON cartridge has been accurately engineered by microfluidic experts to guarantee cell viability with the softest handling and is disposable to remove the risk of cross-contamination present between samples. Also, setting up experiments has never been simpler. The EASY.ON cartridge has been magnetically mounted for simple and rapid loading.
Minimizing cell loss
Video Credit: CYTENA GmbH
It is possible for users to add an extra layer of optimization with Cell Focusing technology, which mildly aligns cells at the center of the dispensing cartridge for excellent cell detection. It provides considerable enhancements while working with unique cell types as this alignment guarantees that cells are ideally captured and quantified. This decreases cell loss and also increases processing speeds.
Benefits designed to optimize workflows
- Double assurance of clonality can be performed
- Greater insights could be offered
- Combined Imager is available
- Quicker than ever before
- Simple to operate and automate
- Users can facilitate their CLD workflow
Image Credit: CYTENA GmbH