Overview
The F.SIGHT™ OMICS enables accurate and precise single-cell dispensing into PCR plates that are available for downstream miniaturization.
Brightfield and fluorescence
- Low-pressure system reduces cell stress
- Bright-field and fluorescent sorting can be done
- Quick start-up and operation, thereby filling a 384-well plate below 8 minutes
- Single-use cartridge needs no cleaning, and decrease the threat of cross contamination
Image Credit: CYTENA GmbH
Precise dispensing enables downstream miniaturization
- Automated offset correction (AOC) tends to adjust droplet trajectory
- Accurate and consistent cell deposition into PCR plates
- Allows downstream miniaturization with lysis buffer volumes below1 μL
Features
Improved, disposable, dispensing, cartridge
Gentle dispensing: Higher cell viability
EASY.ON: magnetic holder
Easy-to-use handling quicker setup time
Cartridges with improved coating
For a wide range of samples: Low cartridge-to-cartridge variation
Cell focusing technology
- Mild alignment of cells in the center of the dispensing cartridge for improved cell detection
- Enhances handling of rare cells
- Processing speeds and cell recovery could be increased
Video Credit: CYTENA GmbH
Automated offset correction (AOC)
- AOC tends to adjust droplet trajectory
- Allowing downstream miniaturization of cell lysis in sub-microliter volumes (<1 µl)
- Guaranteeing accurate and reproducible cell deposition at the center of every well (96/384)
- Extensive documentation: images available for each AOC measurement documenting the offset
Image Credit: CYTENA GmbH
Guaranteed assurance of single-cell dispensation
- Time lapse of single-cell deposition
- Extensive documentation
- Clonality probability is approximately 99%
- Comes with nozzle images for every well-documenting dispensation of single cells
Image Credit: CYTENA GmbH
Processing times
Source: CYTENA GmbH
| |
Time |
| System boot until experiment starts |
<5 min |
| Dispensing (96-well plate) single cells |
~2 min |
| Dispensing (384-well plate) single cells |
~8 min |
| Shutdown and cleaning time |
<5 min |
Key benefits
Bright-field and fluorescence
- Ground-breaking dual-camera system
- Image-based cell detection is possible
- Regulate both cameras separately and freely adapt the power setting for the built-in blue laser
- Concurrent capture of bright-field and fluorescence images at complete resolution
Fast and gentle single cell dispensing
- Low impact on cell integrity or viability
- Just 5 to 80 µl sample volume needed
- Single cells are mildly dispensed into a 96-well plate in just 2 minutes or into a 384-well plate in less than seven minutes
EASY.ON cartridges
- Sole cartridge is magnetically fixed for simple and quick loading
- Contrived by microfluidic experts to guarantee cell viability with the tender handling
- Its single-use design removes the threat of cross-contamination between samples
Compatible with robotic automation workflows
F.SIGHT Omics’ lid opens in an automatic manner, thereby making the quickest single-cell dispenser completely consistent with robotic automation workflows
Intuitive software
- Intuitively developed and quick
- Separate fluorescence experiments could be set up within just minutes
- Users can get isolated full-resolution bright-field and fluorescence imaging together with an overlay image
Cell-focusing technology
- Users can mildly align cells at the center of the dispensing cartridge for excellent cell detection
- Cell morphology has been captured and quantified
- High processing speed and least cell loss; considerable enhancements while functioning with unique cell types