Overview
The F.SIGHT™ 2.0 is known to be the quickest single-cell dispenser along with fluorescent sorting.
Faster than ever before
This can be automated by laborious and time-consuming steps, the F.SIGHT 2.0 considerably simplifies Cell Line Development (CLD), Cell and Gene Therapy, and also Single-cell Omics workflows.
The F.SIGHT 2.0 is the quickest single-cell dispenser that separates both unlabelled and green fluorescent cells. With the patented, highly effective, and smooth single-cell technology, single cells have been dispensed into a 96-well plate in just 2 minutes or a 384-well plate in less than 7 minutes.
Brightfield and fluorescence
The inventive dual-camera system allows users to concurrently capture brightfield and fluorescence information at complete resolution. Users have the potential to control both cameras separately and influence the strong built-in and free adjustable blue laser. Being combined, their high dynamic range envisions even the weakest fluorophores.
Image Credit: CYTENA GmbH
Benefits
- 4× faster compared to the F.SIGHT
- Simple operation via magnetic cartridge mounting
- Gentle cell isolation can be performed
- Intuitive software available
- Consistent with robotic automation
- Accurate and precise single-cell deposition can be done for low volume assays in PCR- and 384-well plates
Application areas
Cell line development
Decreasing timelines in producing clonal cell lines for therapeutic protein production
Single-cell Omics
Accurate isolation of cells and nuclei for plate-based single-cell RNA-sequencing and single-cell multi-omics assays
Gene therapy
Production of stable producer cell lines available for viral vector (AAV) production
Cell therapy and stem cells
Gentle isolation of gene-edited iPSCs
Features
Reducing the risk of cross-contamination
The EASY.ON cartridge has been engineered accurately by microfluidic experts to guarantee cell viability with tender handling and is disposable to remove the threat of cross-contamination between samples. Also, setting up their experiments has never been simpler as the EASY.ON cartridge is magnetically fixed for simple and quick loading.
Minimizing cell loss
Video Credit: CYTENA GmbH
Add an extra layer of optimization with Cell Focusing technology, which mildly aligns cells at the center of the dispensing cartridge for excellent cell detection. It provides considerable enhancements when functioning with rare cell types as this alignment guarantees that cells are ideally captured and quantified, decreasing cell loss and highly increasing processing speeds.
Intuitive software
The all-new software seems to be quick and intuitive. Users can design their individual fluorescence experiments in just minutes. Users get isolated full-resolution brightfield and fluorescence imaging together with an overlay image. All of what is performed is recorded always and saved for analysis and assurance of clonality that could be performed in the future.
Image Credit: CYTENA GmbH