Overview
HUREL® Dog™ is a co-culture made up of non-parenchymal stromal cells and cryopreserved primary dog hepatocytes.
Example Metabolic Activity (nmoles/hr/106 cells). Source: Visikol Inc.
| Substrate |
Enzyme |
Concentration (µM) |
Day 1 |
Day 4 |
Day 8 |
| 7-Ethoxycoumarin |
Phase I |
25 |
0.07 |
0.13 |
0.22 |
| 7-Hydroxycoumarin |
Phase II |
100 |
9.18 |
19.58 |
9.24 |
| 7-Hydroxycoumarin |
Phase III |
100 |
2.5 |
6.9 |
4.11 |
The HUREL PlatinumHeps™ Media supplemented with 10% serum was used to plate cryopreserved primary dog hepatocytes, which were then switched to the HUREL PlatinumHeps™ Basal Media 24 hours after seeding.
The CYP substrate concentrations and metabolite formation, which is expressed as nmoles/hr/106 cells, are listed in the table above. Upon cell delivery on days D1, D4, and D8, all incubations were performed in triplicate and incubated for 60 minutes.
At 37 °C and with 5% CO2, reactions took place in a humid incubator. In preparation for further LC/MS/MS analysis, collected supernatants were kept at –20 °C.
Phase contrast image in a 96-well at a 10× magnification. Image Credit: Visikol Inc.
Phase contrast image in a 96-well at a 10× magnification. Image Credit: Visikol Inc.
Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (CDFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 96-well at 10× magnification. Image Credit: Visikol Inc.
Example culture origin
Dog donor demographics
- Strain: Beagle
- Number of Donors: 1
- Age (years): 5 months
- Gender: Male