Overview
Primary minipig hepatocytes that have been cryopreserved and then co-cultured with non-parenchymal stromal cells make up HUREL® Minipig™.
Example Metabolic Activity (nmoles/hr/106 cells). Source: Visikol Inc.
| Substrate |
Enzyme |
Concentration (µM) |
Day 1 |
Day 8 |
| 7-Ethoxycoumarin |
Phase I (ECOD) |
100 |
0.234 |
.239 |
| 7-Hydroxycoumarin |
Phase II(UGT) |
100 |
88.8 |
104.4 |
| 7-Hydroxycoumarin |
Phase II (SULT) |
100 |
0.980 |
0.836 |
Cryopreserved minipig hepatocytes were thawed, plated on HUREL PlatinumHeps™ Media supplemented with 10% serum, and then switched to HUREL PlatinumHeps™ maintenance Media 24 hours after seeding.
The concentrations of CYP substrates are listed in the table above, along with the rate of metabolite formation in nmoles per hour per million cells. On Days 1 and 8 (the customer day), incubations were performed in triplicate and incubated for 60 minutes.
The reactions took place in a humid incubator with 5% CO2 at 37 °C. Until further LC/MS/MS analysis, collected supernatants were kept at –20 °C.
Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.
Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.
Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (CDFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 24-well at 10× magnification. Image Credit: Visikol Inc.
Example culture origin
Human donor demographics
- Strain: Gottingen
- Number of Donors: 3
- Age(years): –
- Gender: Male