Overview
HUREL® Rat SD™ is a co-culture made up of non-parenchymal stromal cells and cryopreserved primary rat hepatocytes.
Example Metabolic Activity (nmoles/hr/106 cells). Source: Visikol Inc.
| Substrate |
Enzyme |
Concentration (µM) |
Day 1 |
Day 4 |
Day 8 |
| 7-Ethoxycoumarin |
Phase I |
100 |
0.074 |
0.183 |
0.181 |
| 7-Hydroxycoumarin |
Phase II |
100 |
14.380 |
6.640 |
8.460 |
| 7-Hydroxycoumarin |
Phase II |
100 |
3.848 |
2.484 |
1.572 |
Primary rat hepatocytes that had been cryopreserved were thawed, plated on HUREL PlatinumHeps™ Media supplemented with 10% serum, and then switched to HUREL PlatinumHeps™ maintenance Media 24 hours later.
The concentrations of CYP substrates are listed in the table above, and metabolite formation is expressed as nmoles/hr/106 cells. On days D1, D4, and D8, after cell delivery, all incubations were performed in triplicate and incubated for 60 minutes.
The reactions happened in a humid incubator with 5% CO2 at 37 °C. Until a subsequent LC/MS/MS analysis, collected supernatants were kept at –20 °C.
Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.
Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.
Bile canaliculi assayed via 5- (and-6)-carboxy-2’, 7’- dichlorofluorescein diacetate (C- DCFDA) stain at a concentration of 5 μM and imaged in the GFP channel in a 96-well at 10× magnification with filters EX/EM 492-495/512-527 nm. Image Credit: Visikol Inc.
Example culture origin
Rat donor demographics
- Strain: Sprague-Dawley
- Number of Donors: 16
- Age: 2 months
- Gender: Male