Overview
MatTek PermaCell Cell Culture Inserts are sterile tissue culture tools that are perfect for the 3D organotypic culture and cell growth and differentiation. Researchers have more design freedom when using the 8 mm inserts because of their unique ability to work as either standing or hanging inserts.
The inserts can be used with 6-, 12-, and 24-well culture plate formats and are sold in 24-well plates (24 inserts/plate). For purposes like cell attachment, differentiation, organotypic 3D culture, drug transport/permeability, and imaging studies, researchers can choose inserts from a variety of membrane types.
PermaCell 96-well insert plates are connected to create a single, rigid plate that is appropriate for robotic systems in higher throughput applications.
The 96-well insert plates as well as the individual inserts are both made specifically for 3D organotypic tissue culture and are easily adaptable to TEER calculations and other analytical methods.
Features
8 mm inserts
- 24 inserts of 8 mm packaged in 24-well plate
- Hang-top lid for airlifting can be purchased separately for use in 12- or 24-well formats
- Pore size of 0.4 µm or 8 µm, custom pore sizes available
- PTFE, PET, PC membrane, and custom membranes available
- Since PTFE membranes have a high pore density and are transparent in culture, brightfield and fluorescent microscopy can be used to see the cells
Image Credit: MatTek
96-well insert plates
- Pore size of 0.4 µm
- PET high pore density membrane
- Robotics and TEER compatible
- 96-well insert plates connected in a single, rigid tray
- Common (single) well reservoir plate can be purchased separately
- Large access ports to allow for efficient media sampling
- System includes 96-well insert plate, 96-well reservoir plate, and lid
Image Credit: MatTek
It works with the TEER Electrode: Electrode for TEER in PermaCell Cell Culture Insert Plate: STX HTS EVOM™
Specifications
8 mm Insert specifications
Table 1. Source: MatTek
| . |
. |
| Height (including feet) |
8.8 mm |
| Height of Feet |
0.8 mm |
| Outer Diameter |
13.6 mm |
| Inner Diameter |
8.8 mm |
| Membrane Area |
0.6 cm2 |
| Membrane Pore Sizes |
0.4, 8 µm |
| Solvent Compatibility |
Same as polystyrene |
| Sterility |
Gamma Irradiation, shipped sterile in 24-well plates |
| Membrane Types |
Polyethelyene terephthalate (PET), Polytetrafluorethylene (PTFE), Polycarbonate (PC) |
96-well Insert Plate specifications
Table 2. Source: MatTek
| . |
. |
| Receiver Plates |
127.8 x 85.5 mm |
| Well Depth |
12 mm |
| Membrane Diameter |
4.29 mm |
| Membrane Area |
0.11 cm2 |
| Membrane Pore Sizes |
0.4 µm |
| Solvent Compatibility |
Same as polystyrene |
| Sterility |
Gamma Irradiation, shipped sterile in 96-well plates |
| Membrane Types |
Polyethelyene terephthalate (PET) |
Application
Membrane types
- Polycarbonate (PC)
- Polyethylene terephthalate (PET)
- Teflon (PTFE)
- Clear in culture
- Specialty membranes also available
SEM membrane images. A. Polycarbonate (PC) membrane, B. Polyethylene terephthalate (PET) membrane, C. Teflon (PTFE) membrane. Image Credit: MatTek
Coatings and special orders
MatTek provides standard membrane options like PET, PC, and PTFE. PermaCell Insert and Insert Plates enable the direct growth of numerous cell types. The following custom coatings from Advanced BioMatrix are advised for cell types that need an extracellular matrix coating:
- PureCol® Type I Collagen
- Poly-L-Ornithine
- Vitronectin
- Type IV Collagen
- Poly-L-Lysine
- Fibronectin
- Poly-D-Lysine