Overview
The Biotin Collagen Hybridizing Peptide (CHP) from Advanced BioMatrix is a synthetic peptide that has the potential to attach to denatured collagen strands via hydrogen bonding. This could be done both in histology, in vivo, and in vitro (3D cell culture).
CHP’s act as a special tool for determining denatured collagen.
Collagen Hybridizing Peptides (CHP) from Advanced BioMatrix are labeled with 5-FAM (F-CHP)/sulfo-Cyanine3 (R-CHP) for fluorescence detection or biotin (B-CHP) for avidin or streptavidin-mediated detection.
The triple helix is known to be the hallmark protein structure of collagen. At the time of tissue remodeling, the triple helical collagen molecules have been debased by particular proteases (for example, cathepsin K or MMP) and are unfolded at body temperature.
The Collagen Hybridizing Peptide (CHP), a synthetic peptide, can particularly bind to these denatured collagen strands via hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture).
Through the distribution of the structural motif and the Gly-X-Y repeating sequence of natural collagen, CHP has a powerful ability to hybridize along with denatured collagen strands, in a fashion that is alike to a DNA fragment tempering to its complementary DNA strand at the time of PCR.
CHP is known to be an extremely specific probe that has been for open collagen molecules. It has a negligible affinity to integral collagen molecules as a result of the lack of binding sites; it is also inert about non-specific binding due to its hydrophilic and neutral nature.
Source: Advanced BioMatrix
| Parameter, Testing, and Method |
CHP Biotin #5265 |
| Specialty |
Flexible avidin / streptavidin mediated detection options based on needs, allowing non-green fluorescence and HRP methods to avoid background and enhance signal |
| Formula |
C124H181N31O39S |
| Molecular Weight |
2762.01 g/mol |
| Ex/Em |
N/A |
| Synonym |
Collagen Mimetic Peptide, CMP |
| Solubility |
Aqueous Buffers |
| Storage |
-20 °C as powder for long term storage. 4 °C after reconstitution in water. Protect from light. |
| Source |
Synthetic |
| Shelf Life |
Minimum of 6 months from date of receipt |
Storage/Stability
The product is shipped under ambient conditions and has to be stored at –20 °C.
Features
- Relevant to denatured collagens of all subtypes and from all species; binding depending on collagen’s secondary structure rather than particular epitopes
- Ideal for both frozen and paraffin-embedded sections with no requirement for antigen retrieval
- High affinity and peerless specificity available to collagen with basically no nonspecific binding
- Stable in solution under 4 °C, thereby removing the need to aliquot for storage
- A non-antibody method with no species restrictions, consistent for co-staining with any antibody
- Highly informative, trustworthy, and more comfortable than DQ collagen, zymography, TEM, and SHG
- Small size (2% of IgG by MW) allowing facile tissue penetration at the time of whole tissue staining (with no requirement for sectioning)