Overview
Alginate that has been methacrylated and lyophilized is called PhotoAlginate®. It produces 3D hydrogels that can be made in a variety of concentrations and crosslinked with calcium or light to produce different levels of gel stiffness. Other photocrosslinkable ECMs can be combined with PhotoAlginate®.
PhotoAlginate®, a methacrylated alginate from Advanced BioMatrix, is available for use in photocrosslinkable hydrogels or for blending with other photocrosslinkable hydrogels (such as PhotoGel, PhotoCol, or PhotoHA).
When prepared at different concentrations and photocrosslinked to produce different gel stiffnesses, PhotoAlginate® can create 3D gels. Its ability to crosslink with calcium makes PhotoAlginate® special. Users can purchase this item separately (200 mg of lyophilized, sterile powder) or in a kit with a photoinitiator for photocrosslinking.
Table 1. Source: Advanced BioMatrix
| Item |
Catalog Number |
Photoinitiator Included |
| PhotoAlginate |
#5310 |
N/A |
| PhotoAlginate with Irgacure |
#5353 |
Irgacure (365 nm) |
| PhotoAlginate with LAP |
#5354 |
LAP (405 nm) |
| PhotoAginate with Ruthenium |
#5355 |
Ruthenium (400-450 nm) |
The PhotoAlginate® can be stored at 2–10 °C for a month after it has been solubilized.
Table 2. Source: Advanced BioMatrix
| Parameter, Testing, and Method |
Methacrylated Alginate #5310 |
| Sterilization Method |
Filtration |
| Sterility - USP modified |
No growth |
| Form |
Lyophilized Powder |
| Package Size |
200 mg |
| Storage Temperature |
-20 °C |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Shelf Life After Reconstitution |
1 Month |
| Degree of Methacrylation |
> 20% |
| Molecular Weight |
~ 30 kDa |