Overview
This Type IV Collagen is purified using a multi-step procedure after being isolated from the human placenta. The product is supplied as a non-sterile powder containing 5 mg of Type IV collagen per vial. Each product can be purchased with a Certificate of Analysis.
The extracellular basement membranes separating various epithelial and endothelial cells are composed primarily of type IV collagen. It plays a significant role in the dermal-epidermal junction and is primarily present in the lamina densa. It is a heterotrimeric molecule with two chains that resemble Alpha 1 and one chain that resembles Alpha 2.
On surfaces used for tissue culture, type IV collagen is typically applied as a thin coating. The Directions for Use contain detailed instructions. In vitro, this product is typically used as a substrate scaffold to improve cell attachment, adherence, and proliferation.
Many different cell types, including epithelial, endothelial, muscle, and nerve cells, can be cultured using type IV collagen. The use of this product as a substrate for collagenase assays and positive controls is also acceptable.
Source: Advanced BioMatrix
| Parameter, Testing, and Method |
Type IV Collagen Powder #5016 |
| Form |
Powder |
| Package Size |
5 mg |
| Storage Temperature |
-20 °C prior to reconstitution and 2-10 °C after reconstitution |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Shelf Life after reconstitution |
3 months |
| Adventitious Agents |
Human source has been tested and found negative for Hepatitis B, C and immunodeficiency virus-1. |
| Electrophoretic Pattern - Coomassie Blue |
Characteristic |
| Endotoxin (LAL) |
≤ 1.0 EU/mL |
| Cell Attachment Assay |
Pass |
| Source |
Human Placenta |