Overview
Free tyrosine and acryl groups are covalently crosslinked by the water-soluble photoinitiator ruthenium using visible light (400–450 nm). Ruthenium has been tested on a variety of materials, including gelatin, silk fibroin, methacrylated hyaluronic acid, methacrylated collagen type I, and PEGDA.
Features
- For tissue engineering, cell culture, and bioprinting applications where fine-tuning the mechanical properties of the substrate is necessary, the ruthenium visible light photocrosslinking kit is the best choice
- For more than 200 mL of bioinks or hydrogels, the kit includes enough photoinitiator
- Ruthenium is a photoinitiator that utilizes visible light photocrosslinking (400-450 nm) to covalently crosslink free tyrosine and acryl groups
- Ruthenium photoinitiator has been tested on collagen type I, gelatin, silk fibroin, methacrylated hyaluronic acid, methacrylated gelatin, methacrylated collagen type I and PEGDA
- Ruthenium is water soluble and yields better cell cytocompatibility, and crosslinking efficiency
- Increasing Visible light intensity from 3–100 mW/cm2 using Ruthenium did not significantly decrease cell viability from 90%
- Increasing Ruthenium concentration by 10 times (10×) did not decrease cell viability from 90%
- Ruthenium is red/yellow/orange in color and will change the color of the solutions, hydrogels, or printed constructs
Application
- It is believed that this Ruthenium photocrosslinking kit is non-sterile. It is advised to sterile filter the system or add antibiotics to the cell culture setup
- Ruthenium and sodium persulfate must be resuspended in their entirety (separately) in order to be filtered sterilely through tiny 0.2-micron button filters (separately)
- Within two weeks, use the sterile photoinitiator
- As shown in Table 1, the Ruthenium Visible Light Photocrosslinking Kit consists of two parts.
Source: Advanced BioMatrix
| Item |
Catalog Number |
Package Size |
| Ruthenium Photoinitiator |
#5246 |
200 mg |
| Sodium Persulfate Photoinitiator |
#5247 |
1 gram |
Storage/Stability
- The product is shipped at room temperature. Place the kit in a cool, dry place
- Measure out the necessary quantity of powder for solubilization
- Use the ruthenium and sodium persulfate within two weeks of solubilization
- At room temperature, dry powder that has not been dissolved lasts longer than a year