Overview
The CY3 (Red) Collagen Hybridizing Peptide (CHP) is a synthetic peptide that has the potential to specifically adhere to denatured collagen strands via hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). CHPs act as a special tool for determining denatured collagen.
Collagen Hybridizing Peptides (CHP) conjugates from Advanced BioMatrix are labeled with 5-FAM (F-CHP)/sulfo-Cyanine3 (R-CHP) for fluorescence detection, or biotin (B-CHP) for avidin/streptavidin-mediated detection.
Also, the triple helix is known to be the hallmark protein structure of collagen. At the time of tissue remodeling, the triple helical collagen molecules have been degraded by particular proteases (for example, cathepsin K, or MMP) and turn out to be opened at body temperature.
The Collagen Hybridizing Peptide (CHP) is a synthetic peptide that has the potential to particularly bind to such denatured collagen strands via hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture).
By sharing the structural motif and the Gly-X-Y repeating sequence of natural collagen, CHP exhibits a powerful ability to hybridize with denatured collagen strands, anyhow which is similar to a DNA fragment annealing to its complementary DNA strand at the time of PCR.
CHP is known as a specific probe for open collagen molecules: it has an insignificant affinity to intact collagen molecules as a result of the lack of binding sites; it is also inert towards non-specific binding as a result of its hydrophilic and neutral nature.
Source: Advanced BioMatrix
| Parameter, Testing, and Method |
CHP Cy3 #5276 |
| Specialty |
Straight-forward fluore-scence detection in red |
| Formula |
C144H198N33O46S2 |
| Molecular Weight |
3191.44 g/mol |
| Ex/Em |
548 nm / 563 nm |
| Synonym |
Collagen Mimetic Peptide, CMP |
| Solubility |
Aqueous Buffers |
| Storage |
-20 °C as powder for long term storage. 4 °C after reconstitution in water. Protect from light. |
| Source |
Synthetic |
| Shelf Life |
Minimum of 6 months from date of receipt |
Storage/Stability
The product ships on ambient. Store the product at −20 °C.
Features
- Highly informative, convenient, and suitable compared to DQ collagen, zymography, TEM, and SHG
- Ideal for both frozen and paraffin-embedded sections with no requirement for antigen retrieval
- High affinity and peerless specificity to collagen with basically no nonspecific binding
- A non-antibody method with no species restrictions, consistent for co-staining with any antibody
- Small size (2% of IgG by MW) allowing facile tissue penetration at the time of whole tissue staining (with no requirement for sectioning)
- Relevant to denatured collagens of all subtypes obtained and from all species; binding depending on collagen’s secondary structure rather than specific epitopes
- Firm in solution under 4 °C, eliminating the requirement to aliquot for storage