Overview
For extrusion-based FRESH 3D bioprinting, Lifeink® 200 is a type I collagen bioink with a 35 mg/ml concentration. Lifeink® 200 is available as an isotonic, pH-neutral bioink that is ready for cell addition and bioprinting.
It is strongly advised to use the FRESH printing method and print at 2–8 °C when using Lifeink® 200. The effects of the extracellular matrix’s (ECM) mechanical characteristics, such as density and rigidity, on cell development, migration, and morphology can be studied using three-dimensional (3D) gels.
In contrast to 2D systems, 3D environments enable cell extensions to interact simultaneously with integrins on all cell surfaces, activating particular signaling pathways. In 3D versus 2D environments, gel stiffness or rigidity also has a different effect on cell migration.
High Print Resolution, shear-thinning, strong mechanical strength, superior cytocompatibility, the ability to allow for cellular remodeling, and biomimetic properties are all necessary for a bioink to resemble life. Those conditions are met by Lifeink® 200.
Lifeink® 200, a bioink that contains 35 mg/ml of type I collagen, is available from Advanced BioMatrix. Collagen with a physiological salt concentration, Lifeink® 200 is pH neutral. A carefully controlled manufacturing process is used to purify the collagen starting material.
Image Credit: Advanced BioMatrix
Source: Advanced BioMatrix
| Parameter, Testing, and Method |
Lifeink® 200 #5278 |
| Sterilization Method |
Filtration, Aseptic Processing |
| Sterility - USP modified |
No growth |
| Form |
Opaque Gel/Paste |
| Package Size |
5 mL |
| Storage Temperature |
2-8 °C |
| Print Temperature |
2-8 °C |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Endotoxin - LAL |
≤ 10.0 EU/mL |
| pH |
6.9-7.6 |
| Osmolality (mOsmo H2O/kg) |
290-370 |
| Source Material |
Bovine Collagen |
| Electrophoretic Pattern - Coomassie Blue |
Characteristic |
| Collagen Concentration - Biuret |
30-45 mg/mL |
| Collagen Purity - Silver Staining |
≥ 99% |
| Continuous Flow Extrusion |
Pass |